<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"DejaVu LGC Sans";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:#00FF;mso-bidi-language: #00FF" lang="EN-GB">SAD phasing with in-house Cu <i style="mso-bidi-font-style:normal">Kα </i>radiation using barium as anomalous scatterer</span>
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"DejaVu LGC Sans";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:#00FF;mso-bidi-language: #00FF" lang="EN-GB">SAD phasing with in-house Cu <i style="mso-bidi-font-style:normal">Kα </i>radiation using barium as anomalous scatterer</span>
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| Creator |
Dhanasekaran, V
Velmurugan, D |
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| Subject |
Lysozyme
In-house phasing Single wavelength anomalous diffraction Redundancy |
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| Description |
399-405
<span style="mso-fareast-font-family:" dejavu="" lgc="" sans";="" mso-fareast-language:#00ff;mso-bidi-language:#00ff"="" lang="EN-GB">Phasing of lysozyme crystals using co-crystallized barium ions was performed using single-wavelength anomalous diffraction (SAD) method using Cu <i style="mso-bidi-font-style:normal">Kα</i> radiation with in-house source of data collection. As the ion binding sites vary with respect to the pH of the buffer during crystallization, the highly isomorphic forms of lysozyme crystals grown at acidic and alkaline pH were used for the study. Intrinsic sulphur anomalous signal was also utilized with anomalous signal from lower occupancy ions for phasing. The study showed that to solve the structure by SAD technique, 2.8-fold data redundancy was sufficient when barium was used as an anomalous marker in the in-house copper X-ray radiation source for data collection. Therefore, co-crystallization of proteins with barium containing salt can be a powerful tool for structure determination using lab source. </span> |
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| Date |
2011-12-23T10:32:29Z
2011-12-23T10:32:29Z 2011-12 |
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| Type |
Article
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| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13256 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBB Vol.48(6) [December 2011]
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