<i>In vitro</i> propagation and mass scale multiplication of a critically endangered epiphytic orchid, <i style="">Gastrochilus calceolaris </i>(Buch.-Ham ex J.E.Sm.) D.Don.
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
<i>In vitro</i> propagation and mass scale multiplication of a critically endangered epiphytic orchid, <i style="">Gastrochilus calceolaris </i>(Buch.-Ham ex J.E.Sm.) D.Don.
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| Creator |
Pathak, Promila
Piri, Hossein Vij, S P Mahant, K C Chauhan, Shaveta |
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| Subject |
<i style="">Gastrochilus calceolaris</i>
Immature seeds Nutrient media Seed germination Seedling development |
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| Description |
711-716
<i>In vitro</i> asymbiotic seed germination potential of its immature seeds (36 weeks after pollination) of <i>G.</i><i style=""> calceolaris </i>was successfully tested on three different agar gelled nutrient media i.e. Murashige and Skoog (MS), Mitra <i style="">et al. </i>(M) and potato dextrose agar (PDA). Seeds germinated within 15.75±0.75 to 35.75±0.75 days in the three different media. The protocorms developed therefrom subsequently differentiated into first leaf and root primordia, and complete seedlings were obtained within 111.25±1.25 to 141.25±1.25 days on MS and M media. The protocorms, though failed to differentiate further on basal PDA medium, despite repeated subculturings, incorporation of peptone (P; 1gl<sup>-1</sup>), yeast extract (YE; 2 gl<sup>-1</sup>) and coconut water (CW; 20%) in the medium proved beneficial in inducing differentiation, in these germinating entities. Additional use of growth additives (P/YE/CW), in general, favoured better germination, protocorm formation and seedling development. The optimal nutritional combination during seed germination, protocorm growth and multiplication and seedling development was found to be CW (10%) enriched MS medium. |
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| Date |
2011-08-29T06:44:58Z
2011-08-29T06:44:58Z 2011-09 |
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| Type |
Article
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/12618 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJEB Vol.49(09) [September 2011]
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