A comparative study on the interaction of the putative anticancer alkaloids, sanguinarine and chelerythrine, with single- and double-stranded, and heat-denatured DNAs
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
A comparative study on the interaction of the putative anticancer alkaloids, sanguinarine and chelerythrine, with single- and double-stranded, and heat-denatured DNAs
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| Creator |
Basu, Pritha
Kumar, Gopinatha Suresh |
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| Subject |
Chemistry
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| Description |
A detailed investigation on the interaction of two benzophenanthridine alkaloids, sanguinarine (SGR) and chelerythrine (CHL), with the double-stranded (ds), heat-denatured (hd), and single-stranded (ss) DNA was performed by spectroscopy and calorimetry techniques. Binding to the three DNA conformations leads to quenching of fluorescence of SGR and enhancement in the fluorescence of CHL. The binding was cooperative for both of the alkaloids with all the three DNA conformations. The binding constant values of both alkaloids with the ds DNA were in the order of 106 M−1; binding was weak with hd and much weaker to the ss DNA. The fluorescence emission of the alkaloid molecules bound to the ds and hd DNAs was quenched much less compared to those bound to the ss DNA based on competition with the
anionic quencher KI. For both double stranded and heat denatured structures the emission of the bound alkaloid
molecules was polarized significantly and strong energy transfer from the DNA bases to the alkaloid molecules occurred. Intercalation of SGR and CHL to ds, hd, and ss DNA was proved from these fluorescence results. Calorimetric studies suggested that the binding to all DNA conformations was both enthalpy and entropy favored. Both the alkaloids preferred double-helical regions for binding, but SGR was a stronger binder than CHL to all the three DNA structures.
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2690/1/Journal_of_Biomolecular_Structure_and_Dynamics%2C_2015.pdf
Basu, Pritha and Kumar, Gopinatha Suresh (2015) A comparative study on the interaction of the putative anticancer alkaloids, sanguinarine and chelerythrine, with single- and double-stranded, and heat-denatured DNAs. Journal of Biomolecular Structure and Dynamics, 33 (12). pp. 2594-2605. |
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| Relation |
http://dx.doi.org/10.1080/07391102.2014.1002425
http://www.eprints.iicb.res.in/2690/ |
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