Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.
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| Creator |
Priyanka, Anu
Solanki, Vipul Parkesh, Raman Thakur, Krishan Gopal |
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| Subject |
QR Microbiology
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| Description |
Human SIRT7 is an NAD(+) dependent deacetylase, which belongs to sirtuin family of proteins. SIRT7, like other sirtuins has conserved catalytic domain and is flanked by N- and C-terminal domains reported to play vital functional roles. Here, we report the crystal structure of the N-terminal domain of human SIRT7 (SIRT7(NTD) ) at 2.3 Å resolution as MBP-SIRT7(NTD) fusion protein. SIRT7(NTD) adopts three-helical domain architecture and comparative structural analyses suggest similarities to some DNA binding motifs and transcription regulators. We also report here the importance of N- and C-terminal domains in soluble expression of SIRT7. Proteins 2016; 84:1558-1563. © 2016 Wiley Periodicals, Inc.
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| Publisher |
Wiley
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| Date |
2016-10
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/1897/1/67.pdf
Priyanka, Anu and Solanki, Vipul and Parkesh, Raman and Thakur, Krishan Gopal (2016) Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture. Proteins, 84 (10). pp. 1558-63. ISSN 1097-0134 |
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| Relation |
http://onlinelibrary.wiley.com/doi/10.1002/prot.25085/abstract
http://crdd.osdd.net/open/1897/ |
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