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Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh


Field	Value

Title	Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative

Creator	Chopra, Lipsy Singh, Gurdeep Jena, Kautilya Kumar Verma, Himanshu Sahoo, Debendra K

Subject	QR Microbiology

Description	Media composition and environmental conditions were optimized using statistical tools, Plackett Burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate Bacillus sonorensis MT93 showing broad spectrum of antimicrobial activity. Under optimized conditions, MT93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. As oxygen supply is a critical parameter controlling growth and product formation in aerobic bioprocesses and used as a parameter for bioprocess scale up, the effects of oxygen transfer, in terms of volumetric oxygen transfer coefficient (kLa), on production of sonorensin was investigated using optimized medium composition in a bioreactor. Studies on effectiveness of sonorensin against Staphylococcus aureus and Listeria monocytogenes in fruit juice and as a preservative in pasteurized milk demonstrated its potential as a biopreservative in fruit products and shelf life extender of the pasteurized milk.

Publisher	Elsevier Science

Date	2015

Type	Article PeerReviewed

Relation	http://dx.doi.org/10.1016/j.biortech.2014.10.105 http://crdd.osdd.net/open/1815/

Identifier	Chopra, Lipsy and Singh, Gurdeep and Jena, Kautilya Kumar and Verma, Himanshu and Sahoo, Debendra K (2015) Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative. Bioresource Technology, 175. pp. 358-366. ISSN 09608524