Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies
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| Creator |
Chopra, Adity
Rawat, Sanjay Bhalla, Vijayender Suri, C Raman |
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| Subject |
QR Microbiology
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| Description |
Specific immune detection of glycated hemoglobin is still a great challenge owing to the small epitopic difference between Hemoglobin (Hb) and HbA1c. We report a new electrochemical immunoassay format for point of care testing of HbA1c. A conducting self-assembled monolayer of mercaptophenyl boronic acid (MPBA) was used as a capture layer for binding of glycated proteins and ferrocene tagged anti-HbA1c antibody (FcAb) as a tracer molecule on a gold screen printed electrode. Validation of the new HbA1c assay was carried out using 6 clinical samples with known HPLC values and a correlation coefficient of 98 % was observed.
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| Publisher |
Wiley
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1002/elan.201300646
http://crdd.osdd.net/open/1706/ |
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| Identifier |
Chopra, Adity and Rawat, Sanjay and Bhalla, Vijayender and Suri, C Raman (2014) Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies. Electroanalysis, 26 (3). pp. 469-472. ISSN 10400397
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