Genotoxicity of cadmium chloride in the marine gastropod Nerita chamaeleon using comet assay and alkaline unwinding assay
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Sarkar, A.
Bhagat, J. Ingole, B.S. Rao, P.V.S.S.D.P. Markad, V.L. |
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| Date |
2015-02-17T05:15:28Z
2015-02-17T05:15:28Z 2015 |
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| Identifier |
Environmental Toxicology, vol.30(2); 177-187
http://drs.nio.org/drs/handle/2264/4694 |
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| Description |
This paper presents an evaluation of the genotoxic effects of cadmium chloride (CdCl<sub>2</sub>) on marine gastropod, Nerita chamaeleon following the technique of comet assay and the DNA alkaline unwinding assay (DAUA). In this study, the extent of DNA damage in gill cells of N. chamaeleon was measured after in vivo exposure to four different concentrations (10, 25, 50, and 75 mu g/L) of CdCl<sub>2</sub>. In vitro exposure of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>; 1, 10, 25, and 50 mu M) of the gill cells showed a significant increase in the percentage tail DNA, Olive tail moment, and tail length (TL). Significant changes in percentage tail DNA by CdCl<sub>2</sub> exposure were observed in all exposed groups of snails with respect to those in control. Exposure to 75 mu g/L of CdCl<sub>2</sub> produced significant decrease in DNA integrity as measured by DAUA at all duration with respect to control. In vivo exposure to different concentrations of CdCl<sub>2</sub> (10, 25, 50, and 75 mu g/L) to N. chamaeleon showed considerable increase in DNA damage as observed by both alkaline comet assay and the DAUA. The extent of DNA damage in marine gastropods determined by the application of alkaline comet assay and DAUA clearly indicated the genotoxic responses of marine gastropod, N. chamaeleon to a wide range of cadmium concentration in the marine environment
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| Language |
en
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| Publisher |
Wiley
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| Rights |
This is a preprint of an article published in [Environ. Toxicol., vol.30(2); 2015; 177-187]”and an electronic link to the Journal’s WWW site, located at the following Wiley URL: http://onlinelibrary.wiley.com/doi/10.1002/tox.21883/abstract.
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| Subject |
evaluation
marine environment gastropods Nerita chamaeleon |
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| Title |
Genotoxicity of cadmium chloride in the marine gastropod Nerita chamaeleon using comet assay and alkaline unwinding assay
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| Type |
Journal Article
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