<i>In vitro </i>multiple shoot regeneration and plant production in <i>Alysicarpus rugosus</i> DC. var. <i>heyneanus </i>Baker
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive InfoField | Value | |
Creator |
Bhosle, S V
Thengane, R J Thengane, S R |
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Date |
2013-11-11T11:24:33Z
2013-11-11T11:24:33Z 2005-10 |
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Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/23261 |
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Description |
916-920
A protocol for <i>in vitro </i>multiple shoot regeneration and plant production through seedling (shoot tip) culture was established for <i>Alysicarpus rngosus </i>DC. var. <i>heyneanus </i>Baker. Maximum number of adventitious shoots (14.4) per shoot tip explant were initiated after two subcultures on MS solid medium supplemented with IAA (2.85 μ<i>M</i>) plus BAP (2.22 μ<i>M</i>) after 4 weeks. Shoot elongation (3.0-3.5 cm) was achieved on MS medium without any hormones. Stunted shoots elongated on half MS medium without growth hormones. Rooting occurred in MS medium containing IAA (1.14-2.85μ<i>M</i>) alone or in combination with IBA (0.89-2.46μ<i>M</i>) and or NAA (1.07-2.69μ<i>M</i>). Maximum rooting was established in MS medium supplemented with IAA (2.85μ<i>M</i>). The plants were acclimatized successfully with 55% survival in pot containing cocoa peat and sand (1:1). After a month, hardened plants were transferred to pots with manure, garden soil and sand (1:2:1) for further growth and finally planted in field. |
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Language |
en_US
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Publisher |
NISCAIR-CSIR, India
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Relation |
<span style="font-size:12.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:HI">Int Cl<sup>7</sup> A01H</span>
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Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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Source |
IJEB Vol.43(10) [October 2005]
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Subject |
<i>Alysicarpus rugosus</i>
Multiple Shoot regeneration |
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Title |
<i>In vitro </i>multiple shoot regeneration and plant production in <i>Alysicarpus rugosus</i> DC. var. <i>heyneanus </i>Baker
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Type |
Article
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