In vitro cytotoxicity, antimicrobial, and metal-chelating activity of triterpene saponins from tea seed grown in Kangra valley, India
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
In vitro cytotoxicity, antimicrobial, and metal-chelating activity
of triterpene saponins from tea seed grown in Kangra valley, India
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| Creator |
Joshi, Robin
Sood, Swati Dogra, Poonam Mahendru, Madhvi Kumar, Dharmesh Bhangalia, Shalika Pal, Harish Chandra Kumar, Neeraj Bhushan, Shashi Gulati, Arvind Saxena, Ajit Kumar Saxena Gulati, Ashu |
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| Subject |
Plant sciences
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| Description |
This study was undertaken to isolate and
characterize saponins from seeds of Camellia sinensis.
Four triterpene saponins S1, S2, S3, and S4 were isolated by
chromatography on silica (60–120 mesh), followed by
purification on Sep-Pak C-18 columns. The chemical
structures (S1–S4) were elucidated on the basis of 1-D and
2-D NMR. All the saponins show broad-spectrum antifungal
activity against Candida albicans, Issatchenkia
orientalis, Aspergillus flavus, A. niger, A. ochraceous, A.
parasiticus, A. sydowii, and Trichophyton rubrum. The
most susceptible test fungus was T. rubrum inhibited at a
minimum inhibitory concentration of 31.25 lg/ml by all
the four saponins. Cytotoxicity of these saponins was
evaluated by methyl thiazole tetrazolium and sulfo-rhodamine
B assays. The saponins when tested against five
human cancer cells lines, viz., OVCAR-5 (ovarian carcinoma
cells), MCF-7 (human breast adenocarcinoma cells),
PC-3 (human prostate cancer cells), Colo-205 (colorectal
adenocarcinoma cells), and HL-60 (human promyelocytic
leukemia cells) showed high cytotoxicity activity (99 %)
by S1 and S2 on PC-3 cells at concentration of 100 lg/ml.
Similarly, when these saponins were tested against human
PBMCs by lymphocytes proliferation assay, none showed
significant activity. S3 (IC50 = 1.72 mg/ml) showed high
metal-chelating activity at a concentration of 20 mg/ml.
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1331/1/113_In_vitro_cytotoxicity.pdf
Joshi, Robin and Sood, Swati and Dogra, Poonam and Mahendru, Madhvi and Kumar, Dharmesh and Bhangalia, Shalika and Pal, Harish Chandra and Kumar, Neeraj and Bhushan, Shashi and Gulati, Arvind and Saxena, Ajit Kumar Saxena and Gulati, Ashu (2013) In vitro cytotoxicity, antimicrobial, and metal-chelating activity of triterpene saponins from tea seed grown in Kangra valley, India. Journal of Medicinal Chemistry Research. |
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| Relation |
http://ihbt.csircentral.net/1331/
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