Overexpression of CsANR Increased Flavan-3-ols and Decreased Anthocyanins in Transgenic Tobacco
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Overexpression of CsANR Increased Flavan-3-ols and Decreased
Anthocyanins in Transgenic Tobacco
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| Creator |
kumar, Vinay
Yadav , S K |
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| Subject |
Nanotechnology
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| Description |
plants and synthesized by a common biosynthetic
pathway. Anthocyanin reductase (ANR) represents branching-
point enzyme of this pathway converting anthocyanidins
to flavan-3-ols. Since tea contains highest amount of flavonoids,
a cDNA encoding anthocyanin reductase from tea
(CsANR) was overexpressed in transgenic tobacco to check
the influence on anthocyanin and flavan-3-ols. The transgenic
tobacco was confirmed by genomic PCR and expression
of transgene was analyzed through semiquantitative
PCR. Interestingly flowers of transgenic tobacco were light
pink/white in color instead of dark pink in wild tobacco,
documenting the decrease in anthocyanins content. Upon
measurement, flower anthocyanin content was found to be
lesser. While flavan-3-ols (epicatechin and epigallocatechin)
contents were increased in leaf tissue of transgenic lines. The
expressions of other endogenous flavonoid biosynthetic
pathway genes in different floral parts (sepal, petal, stamen,
and carpel) of CsANR overexpressing tobacco as well as wild
tobacco were analyzed. The transcript levels of PAL and CHI
genes were downregulated, while transcript levels of F3H,
FLS, CHS, ANR1, and ANR2 genes were upregulated in all
floral parts of CsANR transgenic plants compared to wild
tobacco. The expressions of DFR and ANS genes were also
spatially modulated in different floral parts due to overexpression
of CsANR. Thus, CsANR overexpression increased
flavan-3-ols and decreased anthocyanin content by modulating
the expressions of various flavonoid biosynthetic
pathway genes in flower of tobacco. These changes might be
responsible for the observed pollen tube in the pollens of
CsANR overexpressing transgenic tobacco when they were
still in the anther before pollination.
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1311/1/87_Overexpression.pdf
kumar, Vinay and Yadav , S K (2013) Overexpression of CsANR Increased Flavan-3-ols and Decreased Anthocyanins in Transgenic Tobacco. Journal of Molecular Biotechnology. |
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| Relation |
http://ihbt.csircentral.net/1311/
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