16S rRNA gene sequence analyses of the metagenome derived from waters of river Mahananda at Siliguri: An approach to understand bacterial diversity
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Mukherjee, Shriparna
Kumar, Dhananjay Nanda, Ashis K Chakraborty, Ranadhir |
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| Date |
2013-03-28T11:08:56Z
2013-03-28T11:08:56Z 2013-01 |
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://hdl.handle.net/123456789/16526 |
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| Description |
80-87
Bacterial community of the sub-Himalayan river Mahananda at Siliguri, India was characterized by amplification and sequencing of 16S rRNA genes. Analysis of almost complete 115 sequences revealed a total of 43 operational taxonomic units (OTUs). Statistical analysis revealed that coverage of the clone library was 81.73%. All sequences had >85% similarity to 16S rRNA gene sequences retrieved from the DNA databases. The sequences largely constituted of the class <i style="mso-bidi-font-style:normal">Proteobacteria </i>(10.43% Alpha subdivision, 50.43% Beta subdivision, 9.56% Gamma subdivision & 14.7% Epsilon subdivision), phylum <i style="mso-bidi-font-style:normal">Bacteroidetes</i> (8.69%) and <i style="mso-bidi-font-style:normal">Firmicutes</i> (5.21%), besides single representative belonging to the candidate division OP 11 (0.86%). 31.3% of the total clones showed high similarity to uncultured bacteria, suggesting the usefulness of this technique in uncovering microbial diversity in river water. Abundance of Comamonads (61.76%) among <i style="mso-bidi-font-style:normal">Betaproteobacteria</i> indicated transition to eutrophic state, confirming borderline healthy state of Mahananda River at Siliguri. The most dominant genus of this clone library was <i style="mso-bidi-font-style:normal">Arcobacter</i>, whose presence in environmental waters being associated with fecal pollution has been confirmed. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJBT Vol.12(1) [January 2013]
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| Subject |
Amplified ribosomal DNA restriction analysis (ARDRA)
Bacterial community Clone library Mahananda river Phylogeny Ribosomal RNA gene |
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| Title |
16S rRNA gene sequence analyses of the metagenome derived from waters of river Mahananda at Siliguri: An approach to understand bacterial diversity
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| Type |
Article
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