Carbon nanotubes — chitosan nanobiocomposite for immunosensor
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Carbon nanotubes — chitosan nanobiocomposite for immunosensor
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| Creator |
Kaushik, Ajeet
Solanki, Pratima R. Pandey, M. K. Kaneto, Keiichi Ahmad, Sharif Malhotra, B. D. |
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| Subject |
Materials Science
Applied Physics/Condensed Matter Physical Chemistry/Chemical Physics Physics |
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| Description |
Carboxylic group functionalized single walled (SW) and multi walled (MW) carbon nanotubes (CNT) have
been incorporated into biopolymer matrix of chitosan (CH) to fabricate nanobiocomposite film onto indium–
tin–oxide (ITO) coated glass plate for co-immobilization of rabbit-immunoglobulin (r-IgGs) and bovine
serum albumin (BSA) to detect ochratoxin-A (OTA). The results of electrochemical studies reveal that
presence of both CNT results in increased electro-active surface area of CH leading to enhanced electron
transport in these nanobiocomposites. Moreover, in CH–SWCNT and CH–MWCNT nanobiocomposites the
availability of NH2/OH group in CH and surface charged CNT also increases loading of the r-IgGs resulting in
enhanced electron transport responsible for improved sensing characteristics. Compared to BSA/r-IgGs/CH–
MWCNT/ITO immunoelectrode, electrochemical response studies of BSA/r-IgGs/CH–SWCNT/ITO immunoelectrode
carried out as a function of OTA concentration exhibits improved linearity as 0.25–6 ng/dL, detection
limit as 0.25 ng/dL, response time as 25 s, and sensitivity as 21 μA ng dL−1cm−2 with the regression
coefficient as 0.998.
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| Publisher |
Elsevier
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| Date |
2010-08-20
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/33/1/24.pdf
Kaushik, Ajeet and Solanki, Pratima R. and Pandey, M. K. and Kaneto, Keiichi and Ahmad, Sharif and Malhotra, B. D. (2010) Carbon nanotubes — chitosan nanobiocomposite for immunosensor. Thin Solid Films, 519 (3). pp. 1160-1166. ISSN 0040-6090 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0040609010011776
http://npl.csircentral.net/33/ |
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