Simultaneous quantification of Amaryllidaceae alkaloids from Zephyranthes grandiflora by UPLC–DAD/ESI-MS/MS
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Simultaneous quantification of Amaryllidaceae alkaloids from Zephyranthes
grandiflora by UPLC–DAD/ESI-MS/MS
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| Creator |
Katoch, Deepali
Kumar , Shiv Kumar, Neeraj Singh , Bikram |
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| Subject |
Natural Product Chemistry
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| Description |
A rapid, simple and sensitive ultra performance liquid chromatography–diode array detection method
(UPLC–DAD) was developed and validated for quantification of four biologically important Amaryllidaceae
alkaloids viz. lycoramine, hamayne, haemanthamine and tortuosine in Zephyranthes grandiflora.
The method employed BEH C18 column (2.1 mm
×100 mm, 1.7 μ m particle size) with linear gradient elution
of acetonitrile and water (0.05% formic acid) in a flow rate of 0.3 mL/min and at λ max 280 nm. Standard
calibration curve for the analytes were linear (r2 ≥
0.9999), precise (intra-day RSDs < 1.33% and inter-day
RSDs < 2.67%) and accurate (97.8–105.3%). The developed method was applied to the quality assessment
of samples collected during different seasons and showed significant variation with an optimum amounts
of these alkaloids in rainy season. The method was also applied for identification of Amaryllidaceae alkaloids
in the plant and overall, seventeen Amaryllidaceae alkaloids of different structural types lycorine,
haemanthamine, galanthamine, narciclasine were characterised. This study provides a qualitative and
quantitative method for analysis of Amaryllidaceae alkaloids.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1246/1/52_Simultaneous_quantification_of_Amaryllidaceae_alkaloids_from_Zephyranthes.pdf
Katoch, Deepali and Kumar , Shiv and Kumar, Neeraj and Singh , Bikram (2012) Simultaneous quantification of Amaryllidaceae alkaloids from Zephyranthes grandiflora by UPLC–DAD/ESI-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 71. pp. 187-192. |
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| Relation |
http://ihbt.csircentral.net/1246/
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