MassWiz: A Novel Scoring Algorithm with Target-Decoy Based Analysis Pipeline for Tandem Mass Spectrometry
IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi
View Archive Info| Field | Value | |
| Title |
MassWiz: A Novel Scoring Algorithm with Target-Decoy Based
Analysis Pipeline for Tandem Mass Spectrometry
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| Creator |
Yadav, Amit Kumar
Kumar, Dhirendra Dash, Debasis |
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| Subject |
BI1 Bioinformatics (General)
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| Description |
Mass spectrometry has made rapid advances in the recent past and has become the preferred method for proteomics. Although many open source algorithms for peptide identification exist, such as X!Tandem and OMSSA, it has majorly been a domain of proprietary software. There is a need for better, freely available, and configurable algorithms that can help in identifying the correct peptides while keeping the false positives to a minimum. We have developed MassWiz, a novel empirical scoring function that gives appropriate weights to major ions, continuity of b-y ions, intensities, and the supporting neutral losses based on the instrument type. We tested MassWiz accuracy on 486,882 spectra from a standard mixture of 18 proteins generated on 6 different instruments downloaded from the Seattle Proteome Center public repository. We compared the MassWiz algorithm with Mascot, Sequest, OMSSA, and X!Tandem at 1% FDR. MassWiz outperformed all in the largest data set (AGILENT XCT) and was second only to Mascot in the other data sets. MassWiz showed good performance in the analysis of high confidence peptides, i.e., those identified by at least three algorithms. We also analyzed a yeast data set containing 106,133 spectra downloaded from the NCBI Peptidome repository and got similar results. The results demonstrate that MassWiz is an effective algorithm for high-confidence peptide identification without compromising on the number of assignments. MassWiz is open-source, versatile, and easily configurable.
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| Date |
2011-03-18
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://openaccess.igib.res.in/123/1/MassWiz%2D%20A%20Novel%20Scoring%20Algorithm%20with%20Target%2DDecoy%20Based%20Analysis%20Pipeline%20for%20Tandem%20Mass%20Spectrometry.pdf
Yadav, Amit Kumar and Kumar, Dhirendra and Dash, Debasis (2011) MassWiz: A Novel Scoring Algorithm with Target-Decoy Based Analysis Pipeline for Tandem Mass Spectrometry. Journal of Proteome Research, 10 (5). pp. 2154-2160. |
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| Relation |
http://openaccess.igib.res.in/123/
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