A thermostable D-hydantoinase isolated from a mesophilic Bacillus sp.AR9.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
A thermostable D-hydantoinase isolated from a mesophilic Bacillus sp.AR9.
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| Creator |
Sharma, R
Vohra, R M |
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| Subject |
QD Chemistry
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| Description |
A thermostable hydantoinase has been characterized from a mesophilic Bacillus sp.AR9. The hydantoinase produced by this Bacillus sp.AR9 is strictly D-specific and is constitutively produced with high yields (4500 U/ml) in this strain. The enzyme is not only alkalo- and thermostable but has a pH and temperature optimum of 9.5 and 65 degrees C, respectively, which is advantageous for the bioconversion of DL-5-monosubstituted-hydantoin derivatives. The enzyme has a half life of 80 minutes at 70 degrees C and loses only 33% of its activity in 4 hr at 60 degrees C. The enzyme has a broad substrate specificity with a maximum of 100% with hydantoin and about 26% with dihydrouracil. Co+ + ions enhance the activity of the enzyme by more than 60%.
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| Publisher |
Elsevier Science/Academic Press
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| Date |
1997-05-19
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/369/1/vohra1997.pdf
Sharma, R and Vohra, R M (1997) A thermostable D-hydantoinase isolated from a mesophilic Bacillus sp.AR9. Biochemical and biophysical research communications, 234 (2). pp. 485-8. ISSN 0006-291X |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0006291X97966596
http://crdd.osdd.net/open/369/ |
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