Gradual depletion of 2,4-D in the culture medium for indirect shoot regeneration from leaf explants of Camellia sinensis (L.) O. Kuntze
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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Title |
Gradual depletion of 2,4-D in the culture medium for indirect shoot
regeneration from leaf explants of Camellia sinensis (L.) O. Kuntze
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Creator |
Sandal, I
Bhattacharya, Amita Kumar, Amit Sharma, Madhu Shanker, Adarsh Ahuja, Paramvir Singh |
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Subject |
Plant sciences
Plant Biotechnology |
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Description |
Adventitious shoot regeneration via callus phase from in vitro leaf explants is reported for the first time in
tea. Callus was obtained on Murashige and Skoog medium supplemented with varied concentrations of 2,4-
dichlorophenoxyacetic acid (2,4-D) (2.5, 5.0, 7.5 and 10.0 mg/l). Rhizogenesis was observed at all concentrations
of 2,4-D. Adventitious shoot buds developed indirectly on leaf explants after prolonged culture
for 16 weeks on medium supplemented with 10.0 mg/l 2,4-D. GC analysis of the medium and the tissues at
different stages of development showed that specific levels of 2,4-D in the tissue were responsible for
morphogenesis. Shoot buds developed on rhizogenic calli, only when 2,4-D declined to undetectable or
negligible concentrations in the tissue probably due to detoxification and metabolism. Alternatively, shoot
buds could also be evoked when rhizogenic calli were transferred to medium supplemented with low
concentration of 2,4-D (1.5 mg/l). The adventitious nature of the shoots was confirmed through
histological studies.
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Date |
2005
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://ihbt.csircentral.net/48/1/2005_Gradual_depletion.pdf
Sandal, I and Bhattacharya, Amita and Kumar, Amit and Sharma, Madhu and Shanker, Adarsh and Ahuja, Paramvir Singh (2005) Gradual depletion of 2,4-D in the culture medium for indirect shoot regeneration from leaf explants of Camellia sinensis (L.) O. Kuntze. Plant Growth Regulation, 47 (2-3). pp. 121-127. |
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Relation |
http://ihbt.csircentral.net/48/
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