Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Malik, S
Sharma, N Sharma, UK Singh, NP Bhushan, S Sharma, M Sinha, AK Ahuja, PS |
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| Subject |
Plant Sciences
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| Description |
This paper presents quantification of five anthraquinone derivatives (emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion) in rhizomes of hardened micro-propagated Rheum emodi plants using high-performance liquid chromatography (HPLC). Aseptic shoot cultures were raised using rhizome buds. Shoot multiplication occurred in both agar gelled and liquid Murashige and Skoog (MS) medium supplemented with 10.0 mu M 6-benzylaminopurine (BAP) and 5.0 mu M indole-3-butyric acid (IBA). Rooted plantlets obtained on plant growth regulator (PGR)-free medium were transferred to soil with 92% survival. HPLC analysis revealed the presence of five anthraquinone derivatives: emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion in rhizomes of tissue culture-raised plants. Only emodin glycoside (1) and chrysophanol glycoside (2) were present in 6-month-old hardened tissue cultured plants. In addition, the other three derivatives (emodin (3), chrysophanol (4) and physcion (5)) were also detected after 9 months. (C) 2010 Elsevier GmbH. All rights reserved.
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| Publisher |
ELSEVIER GMBH, URBAN & FISCHER VERLAGJENAOFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
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| Date |
2011-09-20T12:08:59Z
2011-09-20T12:08:59Z 2010 |
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| Type |
Article
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| Identifier |
JOURNAL OF PLANT PHYSIOLOGY
0176-1617 http://hdl.handle.net/123456789/13587 |
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| Language |
English
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