Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Jaitak, V
Sharma, K Kalia, K Kumar, N Singh, HP Kaul, VK Singh, B |
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| Subject |
Chemistry; Food Science & Technology
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| Description |
Antioxidant activities of the methanol extract, fractions and isolated compounds from the roots of Potentilla fulgens Lodd. were evaluated by three in vitro experiments, namely, ABTS, DPPH, and FRAP assays. PF-2 was characterized as a new biflavanoid and designated as Potifulgene on the basis of NMR and mass spectrum, whereas PF-1 was identified as epicatechin. The activities of aqueous methanolic extract and fractions could be correlated with their respective total phenolic content and compared with standard natural antioxidants such as quercetin, vitamin C and pyrogallol. The root powder of the plant was extracted with methanol/water (80:20) by cold extraction and its extract was further partitioned with ethyl acetate, butanol and water fractions. Among the three fractions (ethyl acetate, butanol and water fraction) and the total aqueous methanolic extract, the butanol fraction exhibited good scavenging response measured in terms of TEAC (mM Trolox equivalent/mg extract). The butanol fraction was found to possess strong antioxidant activity (2.54 +/- 0.69, 2.41 +/- 0.53, 3.57 +/- 0.05 mM Trolox equivalent/mg extract) with ABTS, DPPH and FRAP assays, respectively. The chemical composition of extracts, studied in terms of total polyphenol content (TPC), was found in the range of 20.61 +/- 0.38 to 33.28 +/- 0.11 mg/g gallic acid equivalent. A significant correlation was observed between total polyphenolics and antioxidant activity, indicating participation of phenolics in antioxidant activities of extract and fractions. The antioxidant activity of new billavanoid (Potifulgene) was found to be higher, i.e. 6.85 +/- 0.38, 4.24 +/- 0.41, 5.35 +/- 0.53 than that of epicatechin, 2.13 +/- 0.05, 1.50 +/- 0.02, 1.57 +/- 0.03 with ABTS, DPPH and FRAP assays. (C) 2009 Elsevier Inc. All rights reserved.
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| Publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCESAN DIEGO525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
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| Date |
2011-09-20T12:08:57Z
2011-09-20T12:08:57Z 2010 |
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| Type |
Article
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| Identifier |
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
0889-1575 http://hdl.handle.net/123456789/13578 |
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| Language |
English
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