Synergistic effects of selected Lactic acid bacteria and Fructooligosaccharides in the maintenance of gastrointestinal health.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/11353/
|
|
| Title |
Synergistic effects of selected Lactic acid
bacteria and Fructooligosaccharides in the maintenance of gastrointestinal health.
|
|
| Creator |
Madhu, A. N.
|
|
| Subject |
29 Microbiological food
|
|
| Description |
Foods that promote state of wellbeing, better health and that reduce the risk of diseases
have become popular as consumers are becoming more and more health conscious. The
use of diet to fortify certain gut microflora is a popular aspect of functional food sciences.
In this context probiotics, prebiotics and synbiotics play a significant role. Recent
research in the area of prebiotic oligosaccharides and synbiotic combinations with
probiotics is leading towards targeted development of functional food.
Lactobacillus plantarum CFR 2194 amongst the screened strains was found to utilize
Fructooligosaccharides efficiently and thus was selected for further studies. Short chain
fatty acids resulting from the prebiotic fermentation were characterized. The inhibitory
effects of these metabolites have been proven by the results obtained on reduction of β-
glucuronidase activity. Further, the organism could also inhibit the growth of pathogen,
producing β-glucuronidase. Short chain fatty acids produced by L. plantarum CFR 2194
inhibited the survival of HCT-15 cells with an IC50 value of 196 μg/ml after 24 h of
incubation. Morphological observations by staining with AO/Etbr confirmed that the
death was induced by apoptosis. Increase in the population of cells with sub-G1 DNA
content indicated, an increase in the apoptotic cells, which was confirmed by Annexin VFITC
staining studies. The study also focused on the production of spray-dried synbiotic
powder containing probiotic L. plantarum CFR 2194 and a prebiotic
Fructooligosaccharides. Results have indicated that L. plantarum in combination of
Fructooligosaccharides exhibited more than 80% of survivability. The storage stability
(viability) of the prepared synbiotic powder, at 4 °C ± 2 for 60 days was found to be
greater than 60%. Conventional yogurt starters in conjunction with either probiotic
bacteria L. plantarum CFR 2194, L. fermentum CFR 2192 and/or Fructooligosaccharides
were used for yogurt preparation. Selected probiotic Lactic acid bacteria exhibited greater
viability with better proteolytic and antioxidant properties in the presence of prebiotic
Fructooligosaccharides. In vivo evaluation of the effect of synbiotic spray dried
formulation on DMH induced colon cancer in rats showed an increase in the caecal
Lactobacillus population and significantly reduced the harmful fecal and colon enzyme
(β-glucuronidase and nitroreductase) activities and ammonia concentration. Synbiotic
supplementation significantly influenced the maintenance of antioxidant status and
reduced aberrant crypt foci proliferation and/or progression, thus signifying the
preventive efficacy of synbiotics against colon cancer.
|
|
| Contributor |
Prapulla, S. G.
|
|
| Date |
2012
|
|
| Type |
Thesis
NonPeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Rights |
—
|
|
| Identifier |
http://ir.cftri.com/11353/1/madhu%20final%20thesis.pdf
Madhu, A. N. (2012) Synergistic effects of selected Lactic acid bacteria and Fructooligosaccharides in the maintenance of gastrointestinal health. PhD thesis, University of Mysore. |
|