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The structure of Rv3717 reveals a novel amidase from Mycobacterium tuberculosis

IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi

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Title The structure of Rv3717 reveals a novel amidase from Mycobacterium tuberculosis
 
Creator Kumar, A
Kumar, S
Kumar, D
Mishra, A
Dewangan, RP
Shrivastava, Priyanka
Ramachandran, Srinivasan
Taneja, Bhupesh
 
Subject G1 Genome informatics (General)
 
Description Bacterial N-acetylmuramoyl-L-alanine amidases are cell-wall hydrolases that hydrolyze the bond between N-acetylmuramic acid and L-alanine in cell-wall glycopeptides. Rv3717 of Mycobacterium tuberculosis has been identified as a unique autolysin that lacks a cell-wall-binding domain (CBD) and its structure has been determined to 1.7 Å resolution by the Pt-­SAD phasing method. Rv3717 possesses an [alpha]/[beta]-fold and is a zinc-dependent hydrolase. The structure reveals a short flexible hairpin turn that partially occludes the active site and may be involved in autoregulation. This type of autoregulation of activity of PG hydrolases has been observed in Bartonella henselae amidase (AmiB) and may be a general mechanism used by some of the redundant amidases to regulate cell-wall hydrolase activity in bacteria. Rv3717 utilizes its net positive charge for substrate binding and exhibits activity towards a broad spectrum of substrate cell walls. The enzymatic activity of Rv3717 was confirmed by isolation and identification of its enzymatic products by LC/MS. These studies indicate that Rv3717, an N-acetylmuramoyl-L-alanine amidase from M. tuberculosis, represents a new family of lytic amidases that do not have a separate CBD and are regulated conformationally.
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://openaccess.igib.res.in/176/1/lv5048.pdf
Kumar, A and Kumar, S and Kumar, D and Mishra, A and Dewangan, RP and Shrivastava, Priyanka and Ramachandran, Srinivasan and Taneja, Bhupesh The structure of Rv3717 reveals a novel amidase from Mycobacterium tuberculosis. STRUCTURAL BIOLOGY, D69.
 
Relation http://scripts.iucr.org/cgi-bin/paper?lv5048
http://openaccess.igib.res.in/176/