Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)
IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi
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| Title |
Antagonism of microRNA Function in Zebrafish Embryos
by Using Locked Nucleic Acid Enzymes (LNAzymes)
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| Creator |
Suryawansh, Hemant
Lalwani, Mukesh kumar Ramasamy, Soundhar Rana, Rajiv Scaria, Vinod Sivasubbu, Sridhar Maiti, Souvik |
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| Subject |
CH1 Chemical Biology
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| Description |
MicroRNAs (miRNAs) have crucial functions in many cellular
processes, such as differentiation, proliferation and apoptosis;
aberrant expression of miRNAs has been linked to human diseases,
including cancer. Tools that allow specific and efficient
knockdown of miRNAs would be of immense importance for
exploring miRNA function. Zebrafish serves as an excellent vertebrate model system to understand the functions of miRNAs
involved in a variety of biological processes. We designed and
employed a strategy based on locked nucleic acid enzymes
(LNAzymes) for in vivo knockdown of miRNA in zebrafish embryos.
We demonstrate that LNAzyme can efficiently knockdown
miRNAs with minimal toxicity to the zebrafish embryos.
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://openaccess.igib.res.in/163/1/Suryawanshi%20Lalwani%20et%20al%202012_LNAzyme.pdf
Suryawansh, Hemant and Lalwani, Mukesh kumar and Ramasamy, Soundhar and Rana, Rajiv and Scaria, Vinod and Sivasubbu, Sridhar and Maiti, Souvik Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes). Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes). |
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| Relation |
http://openaccess.igib.res.in/163/
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