Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A
IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi
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| Title |
Proteomic analysis of zebrafish (Danio rerio) embryos exposed
to cyclosporine A
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| Creator |
Ponnudurai, P Ruby
Basak, Trayambak Ahmad, Shadab Bhardwaj, Gourav Chauhan, Rajendra Kumar Singh, Angom Ramcharan Lalwani, Mukesh kumar Sivasubbu, Sridhar Sengupta, Shantanu |
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| Subject |
CH1 Chemical Biology
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| Description |
Cyclosporine A, a potent immunosuppressive agent extensively used to prevent allograft
rejections, is under scrutiny due to severe toxic effects. CsA therapy is often continued during
pregnancy in conditions such as organ transplantations and autoimmune diseases. Herein, we
investigated the effects of CsA on early morphogenesis of zebrafish and identified a spectrum
of proteins whose expression was altered in the drug treated embryos. Time-lapse fluorescence
imaging of germ-line double transgenic zebrafish embryos treated with CsA revealed severe
blood regurgitation in heart chambers, absence of blood circulation in vessels, pericardial and
yolk sac edema. We also observed lack of mature blood vessels and down-regulation of endothelial
markers in CsA treated embryos. Proteomic analysis using 2D-DIGE followed by massspectrometry
led to the identification of 37 proteins whose expression was significantly modulated
in presence of the drug. These proteins were mostly associated with cytoskeletal/structural
assembly, lipid-binding, stress response and metabolism. Furthermore, mRNA expression analysis
of eight proteins and Western blotting of actin revealed consistency between the changes
observed in protein expression and its corresponding mRNA levels. Our findings demonstrate
that CsA administration during early morphogenesis in zebrafish modulates the expression of
some proteins which are known to be involved in important physiological processes.
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://openaccess.igib.res.in/162/1/Ruby%20et%20al%202011.pdf
Ponnudurai, P Ruby and Basak, Trayambak and Ahmad, Shadab and Bhardwaj, Gourav and Chauhan, Rajendra Kumar and Singh, Angom Ramcharan and Lalwani, Mukesh kumar and Sivasubbu, Sridhar and Sengupta, Shantanu Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A. Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A. |
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| Relation |
http://openaccess.igib.res.in/162/
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